, -Alkyl Dicarboxylic Acids: Characterization by Isocratic Anion-Exchange Chromatography
نویسندگان
چکیده
منابع مشابه
Anion exchange chromatography of acids of the citric acid cycle.
The demonstration of the accumulation of citrate in vivo in the presence of fluoroacetate and the specific modification of this effect produced in various organs by the injection of malonate (14) have increased the desirability of isolation and quantitative determination of the acids of the Krebs cycle and related substances. Further, proposed studies involving the use of radioactive tracers re...
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INTRODUCTION Sialic acids comprise a large family of Nand O-substituted neuraminic acids. The amino group of neuraminic acid is linked to either an N-acetyl or N-glycolyl group, which yields N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), respectively. The hydroxyls of both sialic acids are often modified. The biosynthesis, function, and occurrence of sialic acids have ...
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Column chromatography has evolved to provide a rapid and effective alternative to more laborious methods for preparing high-quality DNA, such as CsCl-gradient centrifugation. This unit describes the use of a column made of a unique anion-exchange resin that selectively binds nucleic acids, allowing rapid separation of DNA from contaminating RNA, proteins, carbohydrates, and metabolites. The pro...
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Most phosphoproteomics experiments rely on prefractionation of tryptic digests before online liquid chromatography-mass spectrometry. This study compares the potential and limitations of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) and anion-exchange chromatography (AEX). At a pH higher than 5, phosphopeptides have two negative charges per residue and are well-retained...
متن کاملAcid-catalyzed lactonization of alpha2,8-linked oligo/polysialic acids studied by high performance anion-exchange chromatography.
Recent studies from many laboratories revealed remarkable structural, distributional, and functional diversities of oligo/polysialic acids (OSA/PSA) that exist in organisms ranging from bacteria to man. These diversities are further complicated by the fact that OSA/PSA spontaneously form lactones under even mildly acidic conditions. By using high performance anion-exchange chromatography (HPAEC...
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ژورنال
عنوان ژورنال: Journal of Chromatographic Science
سال: 1999
ISSN: 0021-9665,1945-239X
DOI: 10.1093/chromsci/37.5.150